| URL: | http://www.bq.ub.es/bioqint/arecerca.html |
Personal Adscrit |
| Investigador/a Principal | Marta Cascante Serratosa |
| Personal de plantilla | Josep Joan Centelles Serra |
| Personal de support | Ursula Valls Benavides |
| Col.laboradors | Esther Aguilar Fadó, Gema Alcarraz Vizán, Adrián Benito Mauricio, Roldán Cortés Giráldez, Pedro de Atauri Carulla, Santiago Díaz Moralli, Shirley Guzmán Avilés, Igor Marín De Mas, Silvia Marín Martínez, Aníbal Miranda , Susana Sánchez Tena, Míriam Zanuy Porquet |
| Visitants | Vitaly Selivanov |
Línies de recerca |
El nostre grup desenvolupa una recerca interdisciplinària combinant les tècniques de la bioquímica, la biologia cel•lular i molecular i la bioinformàtica per a l’anàlisi integrada dels sistemes biològics.
Teràpia del càncer
- Estudi de les diferències en la regulació del metabolisme de la glucosa en cèl•lules normals i en diferents línies tumorals
- Disseny de teràpies antitumorals basades en la inhibició de diferents etapes de la via de les pentoses fosfat
- Caracterització de l’efecte de potencials fàrmacs antitumorals a nivell cel•lular i metabòlic
Identificació de molècules d’interès en les indústries farmacològica, cosmètica i d’alimentació a partir de productes naturals
- Estudi de les propietats antitumorals i antioxidants de polifenols bioactius extrets a partir de subproductes de la indústria agroalimentària i forestal
Anàlisi del control i la regulació de sistemes bioquímics amb finalitat biomèdica o biotecnològica
- Modelatge de les rutes metabòliques i predicció dels resultats de la manipulació de la via per enginyeria genètica amb finalitat industrial o terapèutica
- Desenvolupament de software en l’entorn Mathematica(R) per a l’anàlisi i modelatge de vies metabòliques
- Anàlisi del disseny i optimització de les senyals de regulació de les vies metabòliques
- Desenvolupament teòric i aplicació experimental del Metabolic Control Analysis (MCA) i de la Biochemical Systems Theory (BST)
- Modelització de sistemes bioquímics utilitzant mètodes cinètics o estocàstics
- Estudi del paper de les interaccions enzim-enzim i de les unions biopolímers-enzims
Metabolòmica i fluxòmica
- Mesura de metabòlits per GC/MS i LC/MS en mostres d’interès biomèdic, biotecnològic i/o industrial
- Desenvolupament i aplicació de software per a la quantificació de fluxos metabòlics utilitzant informació a nivell metabolòmic i cinètic
- Caracterització de les adaptacions metabòliques que acompanyen:
· la diferenciació cel·lular
· la mort cel·lular
· la tumorogènesis
· la transformació per oncogens
· el tractament amb fàrmacs |
Publicacions relevantsLlista completa |
- Vizán P, Sánchez-Tena S, Alcarraz-Vizán G, Soler M, Messeguer R, Pujol MD, Lee WN, Cascante M. Characterization of the metabolic changes underlying growth factor angiogenic activation: identification of new potential therapeutic targets. (2009) . Carcinogenesis 30(6):946-52. (PubMedId: 19369582
- Pachón-Peña G, Reyes-Zurita FJ, Deffieux G, Azqueta A, Cerain AL, Centelles JJ, Creppy EE, Cascante M. Antiproliferative effect of flavomannin-6,6’-dimethylether from Tricholoma equestre on Caco-2 cells. (2009) . Toxicology [Epub ahead of print]. (PubMedId: 19686795
- Santos RC, Salvador JA, Marín S, Cascante M.
. Novel semisynthetic derivatives of betulin and betulinic acid with cytotoxic activity. (2009) Bioorg Med Chem. 17(17):6241-50. (PubMedId: 19674909
- Rodríguez-Prados JC, de Atauri P, Maury J, Ortega F, Portais JC, Chassagnole C, Acerenza L, Lindley ND, Cascante M
. In silico strategy to rationally engineer metabolite production: A case study for threonine in Escherichia coli. (2009) Biotechnol Bioeng. 103(3):609-20 (PubMedId: 19219914
- Reyes-Zurita FJ, Rufino-Palomares EE, Lupiáñez JA, Cascante M. Maslinic acid, a natural triterpene from Olea europaea L., induces apoptosis in HT29 human colon-cancer cells via the mitochondrial apoptotic pathway. (2009) Cancer Lett. 273(1):44-54 (PubMedId: 18790561
- Vizán P, Alcarraz-Vizán G, Díaz-Moralli S, Solovjeva ON, Frederiks WM, Cascante M. Modulation of pentose phosphate pathway during cell cycle progression in human colon adenocarcinoma cell line HT29. (2009) Int J Cancer. 124(12):2789-96 (PubMedId: 19253370
- Vizán P, Alcarraz-Vizán G, Díaz-Moralli S, Solovjeva ON, Frederiks WM, Cascante M. Modulation of pentose phosphate pathway during cell cycle progression in human colon adenocarcinoma cell line HT29. (2009) Int J Cancer. 124(12):2789-96 (PubMedId: 19253370
- Touriño S, Fuguet E, Jáuregui O, Saura-Calixto F, Cascante M, Torres JL
. High-resolution liquid chromatography/electrospray ionization time-of-flight mass spectrometry combined with liquid chromatography/electrospray ionization tandem mass spectrometry to identify polyphenols from grape antioxidant dietary fiber. (2008) . Rapid Commun Mass Spectrom 22(22):3489-500 (PubMedId: 18853405
- Cascante M, Marin S
. Metabolomics and fluxomics approaches.
(2008) . Essays Biochem 45:67-81. Review (PubMedId: 18793124
- Ortega F, Cascante M, Acerenza L.. Kinetic properties required for sustained or paradoxical control of metabolic fluxes under large changes in enzyme activities. (2008) . J Theor Biol. 252(3):569-73. (PubMedId: 18045618
- Vizán, P.; Mazurek, S. and Cascante, M. Robust metabolic adaptation underlying tumor progression (2008) . Metabolomics 4:1-12 (PubMedId:
- Lizárraga D, Touriño S, Reyes-Zurita FJ, de Kok TM, van Delft JH, Maas LM, Briedé JJ, Centelles JJ, Torres JL, Cascante M
. Witch hazel (Hamamelis virginiana) fractions and the importance of gallate moieties--electron transfer capacities in their antitumoral properties. (2008) J Agric Food Chem. 56(24):11675-82 (PubMedId: 19035659
- Selivanov VA, Zeak JA, Roca J, Cascante M, Trucco M, Votyakova TV. The role of external and matrix ph in mitochondrial reactive oxygen species generation. (2008) J Biol Chem. 283(43):29292-300 (PubMedId: 18687689
- Pachon G, Rasoanaivo H, Azqueta A, Rakotozafy JC, Raharisololalao A, De Cerain AL, De Lapuente J, Borras M, Moukha S, Centelles JJ, Creppy EE, Cascante M. Anticancer effect of a new benzophenanthridine isolated from Zanthoxylum madagascariense (Rutaceline) (2007) . In Vivo 21(2):417-22 (PubMedId: 17436597
- Centelles J. J, Ramos-Montoya A, Lim S, Bassilian S, Boros L. G, Marín S, Cascante M, Lee W-N P. Metabolic profile and quantification of deoxyribose synthesis pathways in HepG2 cells (2007) . Metabolomics DOI: 10.1007/s11306-006-0049-8 (PubMedId:
- Vizan P, Alcarraz-Vizan G, Diaz-Moralli S, Rodriguez-Prados JC, Zanuy M, Centelles JJ, Jauregui O, Cascante M. Quantification of Intracellular Phosphorylated Carbohydrates in HT29 Human Colon Adenocarcinoma Cell Line Using Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry (2007) Anal Chem. 79(13):5000-5005 (PubMedId: 17523595
- Selivanov V, Krause S, Roca J, Cascante M. Modeling of Spatial Metabolite Distributions in the Cardiac Sarcomere (2007) Biophys J. 92(10):3492-500 (PubMedId: 17325002
- Lizarraga D, Lozano C, Briede JJ, van Delft JH, Tourino S, Centelles JJ, Torres JL, Cascante M. The importance of polymerization and galloylation for the antiproliferative properties of procyanidin-rich natural extracts (2007) FEBS J. 274(18):4802-4811 (PubMedId: 17824958
- Cespedes MV, Sancho FJ, Guerrero S, Parreno M, Casanova I, Pavon MA, Marcuello E, Trias M, Cascante M, Capella G, Mangues R. K-Ras Asp12 mutant neither interacts with Raf, nor signals through Erk, and is less tumorigenic than K-Ras Val12 (2006) . Carcinogenesis 27(11):2190-200. (PubMedId: 16679305
- Selivanov VA, Marin S, Lee PW, Cascante M. Software for dynamic analysis of tracer-based metabolomic data: estimation of metabolic fluxes and their statistical analysis (2006) Bioinformatics. 22(22):2806-12. (PubMedId: 17000750
- de Atauri P, Ramirez MJ, Kuchel PW, Carreras J, Cascante M. Metabolic homeostasis in the human erythrocyte: in silico analysis. (2006) Biosystems. 83(2-3):118-24. (PubMedId: 16236423
- Ortega F, Garces JL, Mas F, Kholodenko BN, Cascante M. Bistability from double phosphorylation in signal transduction. Kinetic and structural requirements. (2006) FEBS J. 273(17):3915-26. Review. (PubMedId: 16934033
- Lozano C, Julia L, Jimenez A, Tourino S, Centelles JJ, Cascante M, Torres JL. Electron-transfer capacity of catechin derivatives and influence on the cell cycle and apoptosis in HT29 cells.
(2006) FEBS J. 273(11):2475-86. (PubMedId: 16704421
- Reyes FJ, Centelles JJ, Lupianez JA, Cascante M. (2alpha,3beta)-2,3-Dihydroxyolean-12-en-28-oic acid, a new natural triterpene from Olea europea, induces caspase dependent apoptosis selectively in colon adenocarcinoma cells. (2006) FEBS Lett. 580(27):6302-10. (PubMedId: 17083937
- Ramos-Montoya A, Lee WN, Bassilian S, Lim S, Trebukhina RV, Kazhyna MV, Ciudad CJ, Noe V, Centelles JJ, Cascante M. Pentose phosphate cycle oxidative and nonoxidative balance: A new vulnerable target for overcoming drug resistance in cancer. (2006) Int J Cancer. 119(12):2733-41 (PubMedId: 17019714
- Rodriguez-Caso C, Montanez R, Cascante M, Sanchez-Jimenez F, Medina MA. Mathematical modeling of polyamine metabolism in mammals. (2006) J Biol Chem. 281(31):21799-812. (PubMedId: 16709566
- Boren J, Ramos-Montoya A, Bosch KS, Vreeling H, Jonker A, Centelles JJ, Cascante M, Frederiks WM. In situ localization of transketolase activity in epithelial cells of
different rat tissues and subcellularly in liver parenchymal cells. (2006) J Histochem Cytochem. 54(2):191-9. Epub 2005 Aug 22. (PubMedId: 16116031
- Selivanov VA, Meshalkina LE, Solovjeva ON, Kuchel PW, Ramos-Montoya A, Kochetov GA, Lee PW, Cascante M. Rapid simulation and analysis of isotopomer distributions using
constraints based on enzyme mechanisms: an example from HT29 cancer cells. (2005) Bioinformatics. 21(17):3558-64. (PubMedId: 16002431
- Vizan P, Boros LG, Figueras A, Capella G, Mangues R, Bassilian S, Lim S, Lee WN, Cascante M. K-ras codon-specific mutations produce distinctive metabolic phenotypes in
NIH3T3 mice [corrected] fibroblasts. (2005) Cancer Res. 65(13):5512-5. (PubMedId: 15994921
- Lozano C, Torres JL, Julia L, Jimenez A, Centelles JJ, Cascante M. Effect of new antioxidant cysteinyl-flavanol conjugates on skin cancer
cells. (2005) FEBS Lett. 579(20):4219-25. (PubMedId: 16051220
- Tourino S, Selga A, Jimenez A, Julia L, Lozano C, Lizarraga D, Cascante M, Torres JL. Procyanidin fractions from pine (Pinus pinaster) bark: radical scavenging
power in solution, antioxidant activity in emulsion, and antiproliferativeeffect in melanoma cells. (2005) J Agric Food Chem. 53(12):4728-35. (PubMedId: 15941307
- Frederiks WM, van Marle J, van Oven C, Comin-Anduix B, Cascante M. Improved localization of glucose-6-phosphate dehydrogenase activity in
cells with 5-cyano-2,3-ditolyl-tetrazolium chloride as fluorescent redoxdye reveals its cell cycle-dependent regulation. (2005) J Histochem Cytochem. 54(1):47-52. (PubMedId: 16046670
- Marin S, Lee WN, Bassilian S, Lim S, Boros LG, Centelles JJ, FernAndez-Novell JM, Guinovart JJ, Cascante M. Dynamic profiling of the glucose metabolic network in fasted rat
hepatocytes using [1,2-13C2]glucose. (2004) Biochem J. 381(Pt 1):287-94. (PubMedId: 15032751
- Selivanov VA, Puigjaner J, Sillero A, Centelles JJ, Ramos-Montoya A, Lee PW, Cascante M. An optimized algorithm for flux estimation from isotopomer distribution in
glucose metabolites. (2004) Bioinformatics. 20(18):3387-97. (PubMedId: 15256408
- Lee WN, Guo P, Lim S, Bassilian S, Lee ST, Boren J, Cascante M, Go VL, Boros LG. Metabolic sensitivity of pancreatic tumour cell apoptosis to glycogen
phosphorylase inhibitor treatment. (2004) Br J Cancer. 91(12):2094-100. (PubMedId: 15599384
- Goldstein BN, Ermakov G, Centelles JJ, Westerhoff HV, Cascante M. What makes biochemical networks tick? (2004) Eur J Biochem. 271(19):3877-87. (PubMedId: 15373833
- Orosz F, Wagner G, Ortega F, Cascante M, Ovadi J. Glucose conversion by multiple pathways in brain extract: theoretical and
experimental analysis. (2003) Biochem Biophys Res Commun. 309(4):792-7. (PubMedId: 13679042
- Sakamoto N, de Atauri P, Cascante M. Characterization of the transit and transition times for a pathway unit of
Michaelis-Menten mechanism. (2003) Biochim Biophys Acta. 1623(1):6-12. (PubMedId: 12957711
- Vera J, de Atauri P, Cascante M, Torres NV. Multicriteria optimization of biochemical systems by linear programming:
application to production of ethanol by Saccharomyces cerevisiae. (2003) Biotechnol Bioeng. 83(3):335-43. (PubMedId: 12783489
- Boros LG, Steinkamp MP, Fleming JC, Lee WN, Cascante M, Neufeld EJ. Defective RNA ribose synthesis in fibroblasts from patients with
thiamine-responsive megaloblastic anemia (TRMA). (2003) Blood. 102(10):3556-61. (PubMedId: 12893755
- Maher AD, Kuchel PW, Ortega F, de Atauri P, Centelles J, Cascante M. Mathematical modelling of the urea cycle. A numerical investigation into
substrate channelling. (2003) Eur J Biochem. 270(19):3953-61. (PubMedId: 14511377
- Matito C, Mastorakou F, Centelles JJ, Torres JL, Cascante M. Antiproliferative effect of antioxidant polyphenols from grape in murine
Hepa-1c1c7. (2003) Eur J Nutr. 42(1):43-9. (PubMedId: 12594540
- Marin S, Chiang K, Bassilian S, Lee WN, Boros LG, Fernandez-Novell JM, Centelles JJ, Medrano A, Rodriguez-Gil JE, Cascante M. Metabolic strategy of boar spermatozoa revealed by a metabolomic
characterization. (2003) FEBS Lett. 554(3):342-6. (PubMedId: 14623091
- Boren J, Lee WN, Bassilian S, Centelles JJ, Lim S, Ahmed S, Boros LG, Cascante M. The stable isotope-based dynamic metabolic profile of butyrate-induced
HT29 cell differentiation. (2003) J Biol Chem. 278(31):28395-402. (PubMedId: 12750369
- Torres JL, Varela B, Garcia MT, Carilla J, Matito C, Centelles JJ, Cascante M, Sort X, Bobet R. Valorization of grape (Vitis vinifera) byproducts. Antioxidant and
biological properties of polyphenolic fractions differing in procyanidincomposition and flavonol content. (2002) J Agric Food Chem. 50(26):7548-55. (PubMedId: 12475269
- Lozano C, Cascante M, Torres JL. Cation-exchange micropreparative separation of galloylated and
non-galloylated sulphur conjugated catechins. (2002) J Chromatogr A. 973(1-2):229-34. (PubMedId: 12437183
- De Atauri P, Acerenza L, Kholodenko BN, De La Iglesia N, Guinovart JJ, Agius L, Cascante M. Occurrence of paradoxical or sustained control by an enzyme when
overexpressed: necessary conditions and experimental evidence with regardto hepatic glucokinase. (2001) Biochem J. 355(Pt 3):787-93. (PubMedId: 11311143
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