Personal Adscrit |
| Investigador/a Principal | Albert Boronat Margosa |
| Personal de plantilla | Montserrat Busquets Abió, Narciso Campos Martinez, Santiago Imperial Rodenas, Luisa María Lois Rojas, Manuel Rodriguez Concepcion |
| Col.laboradors | Djedoux-Maxime Angaman , Albert Cairo Calzada, Sergi Ferrero Torrero, Victor Giménez Oya, Francesc Hernández Gras, Anna Orozco García, Rocco Petrizzo , Gabriela Puntes del Rey, Antía Rodríguez Villalón, Irma Roig Villanova, Josep Seguí Borrell, Eva Urós Gracia |
Línies de recerca |
Durant els últims 15 anys el nostre grup ha estat estudiant la biosíntesi d’isoprenoides en plantes superiors. Més recentment, el nostre interès s’ha extès a la caracterització d’aquesta ruta biosintètica en microorganismes. Molts isoprenoides tenen papers essencials en el creixement i desenvolupament. Addicionalment, alguns isoprenoides tenen interès degut a les seves aplicacions biotecnològiques en la indústria farmacèutica, alimentària i cosmètica, com els fitosterols (agents hipocolesterolèmics), els carotenoides o el taxol (antitumoral). Les plantes contenen dues rutes per a la síntesi de precursors estructurals dels isoprenoides: la ruta del mevalonat, localitzada dins el citosol/ER, i la recentment descoberta ruta del metileritrol 4-fosfat (MEP), situada als plàstids. La majoria dels microorganismes, inclosos els bacteris patogènics, sintetitzen isoprenoides exclusivament a través de la ruta MEP. Tenint en compte que aquesta ruta essencial no és present als animals, els enzims de la tuta MEP representen dianes potencials pel desenvolupament de nous antibiòtics i herbicides. En aquest context, un dels nostres objectius principals és el d’obtenir nous punts de vista sobre la organització i regulació d’aquesta ruta metabòlica complexa, amb l’objectiu a llarg plaç d’utilitzar la informació obtinguda per emprendre estratègies d’enginyeria metabòlica orientades a l’increment de la producció d’isoprenoides amb interès farmacèutic i alimentari, i també al disseny i desenvolupament de nous antibiòtics.
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Publicacions relevantsLlista completa |
- Sauret-Gueto S, Uros EM, Ibanez E, Boronat A, Rodriguez-Concepcion M. A mutant pyruvate dehydrogenase E1 subunit allows survival of Escherichia
coli strains defective in 1-deoxy-D-xylulose 5-phosphate synthase. (2006) FEBS Lett. 580(3):736-40. Epub 2006 Jan 9. (PubMedId: 16414046
- Sauret-Gueto S, Botella-Pavia P, Flores-Perez U, Martinez-Garcia JF, San Roman C, Leon P, Boronat A, Rodriguez-Concepcion M. Plastid cues post-transcriptionally regulate the accumulation of key
enzymes of the methylerythritol phosphate pathway in Arabidopsis. (2006) Plant Physiol. . (PubMedId: 16531478
- Bernal C, Palacin C, Boronat A, Imperial S. A colorimetric assay for the determination of
4-diphosphocytidyl-2-C-methyl-D-erythritol 4-phosphate synthase activity. (2005) Anal Biochem. 337(1):55-61. (PubMedId: 15649375
- Bernal C, Mendez E, Terencio J, Boronat A, Imperial S. A spectrophotometric assay for the determination of
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase activity. (2005) Anal Biochem. 340(2):245-51. (PubMedId: 15840497
- Vidal-Mas J, Busquets M, Manresa A. Cloning and expression of a lipoxygenase from Pseudomonas aeruginosa 42A2. (2005) Antonie Van Leeuwenhoek. 87(3):245-51. (PubMedId: 15803390
- Leivar P, Gonzalez VM, Castel S, Trelease RN, Lopez-Iglesias C, Arro M, Boronat A, Campos N, Ferrer A, Fernandez-Busquets X. Subcellular localization of Arabidopsis
3-hydroxy-3-methylglutaryl-coenzyme A reductase. (2005) Plant Physiol. 137(1):57-69. Epub 2004 Dec 23. (PubMedId: 15618432
- Busquets M, Deroncele V, Vidal-Mas J, Rodriguez E, Guerrero A, Manresa A. Isolation and characterization of a lipoxygenase from Pseudomonas 42A2
responsible for the biotransformation of oleic acid into ( S )-( E)-10-hydroxy-8-octadecenoic acid. (2004) Antonie Van Leeuwenhoek. 85(2):129-39. (PubMedId: 15028873
- Besumbes O, Sauret-Gueto S, Phillips MA, Imperial S, Rodriguez-Concepcion M, Boronat A. Metabolic engineering of isoprenoid biosynthesis in Arabidopsis for the
production of taxadiene, the first committed precursor of Taxol. (2004) Biotechnol Bioeng. 88(2):168-75. (PubMedId: 15449291
- Rodriguez-Concepcion M, Fores O, Martinez-Garcia JF, Gonzalez V, Phillips MA, Ferrer A, Boronat A. Distinct light-mediated pathways regulate the biosynthesis and exchange of
isoprenoid precursors during Arabidopsis seedling development. (2004) Plant Cell. 16(1):144-56. Epub 2003 Dec 5. (PubMedId: 14660801
- Botella-Pavia P, Besumbes O, Phillips MA, Carretero-Paulet L, Boronat A, Rodriguez-Concepcion M. Regulation of carotenoid biosynthesis in plants: evidence for a key role
of hydroxymethylbutenyl diphosphate reductase in controlling the supply ofplastidial isoprenoid precursors. (2004) Plant J. 40(2):188-99. (PubMedId: 15447646
- Manzano D, Fernandez-Busquets X, Schaller H, Gonzalez V, Boronat A, Arro M, Ferrer A. The metabolic imbalance underlying lesion formation in Arabidopsis
thaliana overexpressing farnesyl diphosphate synthase (isoform 1S) leadsto oxidative stress and is triggered by the developmental decline ofendogenous HMGR activity. (2004) Planta. 219(6):982-92. Epub 2004 Jun 15. (PubMedId: 15605175
- Sauret-Gueto S, Ramos-Valdivia A, Ibanez E, Boronat A, Rodriguez-Concepcion M. Identification of lethal mutations in Escherichia coli genes encoding
enzymes of the methylerythritol phosphate pathway. (2003) Biochem Biophys Res Commun. 307(2):408-15. (PubMedId: 12859972
- Wolff M, Seemann M, Tse Sum Bui B, Frapart Y, Tritsch D, Garcia Estrabot A, Rodriguez-Concepcion M, Boronat A, Marquet A, Rohmer M. Isoprenoid biosynthesis via the methylerythritol phosphate pathway: the
(E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase (LytB/IspH) fromEscherichia coli is a [4Fe-4S] protein. (2003) FEBS Lett. 541(1-3):115-20. (PubMedId: 12706830
- Rodriguez-Concepcion M, Querol J, Lois LM, Imperial S, Boronat A. Bioinformatic and molecular analysis of hydroxymethylbutenyl diphosphate
synthase (GCPE) gene expression during carotenoid accumulation in ripeningtomato fruit. (2003) Planta. 217(3):476-82. Epub 2003 Apr 30. (PubMedId: 12721677
- Seemann M, Bui BT, Wolff M, Tritsch D, Campos N, Boronat A, Marquet A, Rohmer M. Isoprenoid biosynthesis through the methylerythritol phosphate pathway:
the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase (GcpE) is a[4Fe-4S] protein. (2002) Angew Chem Int Ed Engl. 41(22):4337-9. (PubMedId: 12434382
- Querol J, Campos N, Imperial S, Boronat A, Rodriguez-Concepcion M. Functional analysis of the Arabidopsis thaliana GCPE protein involved in
plastid isoprenoid biosynthesis. (2002) FEBS Lett. 514(2-3):343-6. (PubMedId: 11943178
- Puigventos F, Riera M, Delibes C, Penaranda M, de la Fuente L, Boronat A. [Adherence to antiretroviral drug therapy. A systematic review] (2002) Med Clin (Barc). 119(4):130-7. (PubMedId: 12106524
- Kohle A, Sommer S, Yazaki K, Ferrer A, Boronat A, Li SM, Heide L. High level expression of chorismate pyruvate-lyase (UbiC) and HMG-CoA
reductase in hairy root cultures of Lithospermum erythrorhizon. (2002) Plant Cell Physiol. 43(8):894-902. (PubMedId: 12198192
- Masferrer A, Arro M, Manzano D, Schaller H, Fernandez-Busquets X, Moncalean P, Fernandez B, Cunillera N, Boronat A, Ferrer A. Overexpression of Arabidopsis thaliana farnesyl diphosphate synthase
(FPS1S) in transgenic Arabidopsis induces a cell death/senescence-likeresponse and reduced cytokinin levels. (2002) Plant J. 30(2):123-32. (PubMedId: 12000449
- Carretero-Paulet L, Ahumada I, Cunillera N, Rodriguez-Concepcion M, Ferrer A, Boronat A, Campos N. Expression and molecular analysis of the Arabidopsis DXR gene encoding
1-deoxy-D-xylulose 5-phosphate reductoisomerase, the first committedenzyme of the 2-C-methyl-D-erythritol 4-phosphate pathway. (2002) Plant Physiol. 129(4):1581-91. (PubMedId: 12177470
- Rodriguez-Concepcion M, Boronat A. Elucidation of the methylerythritol phosphate pathway for isoprenoid
biosynthesis in bacteria and plastids. A metabolic milestone achievedthrough genomics. (2002) Plant Physiol. 130(3):1079-89. (PubMedId: 12427975
- Querol J, Besumbes O, Lois LM, Boronat A, Imperial S. A fluorometric assay for the determination of 1-deoxy-D-xylulose
5-phosphate synthase activity. (2001) Anal Biochem. 296(1):101-5. (PubMedId: 11520037
- Querol J, Rodriguez-Concepcion M, Boronat A, Imperial S. Essential role of residue H49 for activity of Escherichia coli
1-deoxy-D-xylulose 5-phosphate synthase, the enzyme catalyzing the firststep of the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoidSynthesis. (2001) Biochem Biophys Res Commun. 289(1):155-60. (PubMedId: 11708793
- Campos N, Rodriguez-Concepcion M, Sauret-Gueto S, Gallego F, Lois LM, Boronat A. Escherichia coli engineered to synthesize isopentenyl diphosphate and
dimethylallyl diphosphate from mevalonate: a novel system for the geneticanalysis of the 2-C-methyl-d-erythritol 4-phosphate pathway for isoprenoidbiosynthesis. (2001) Biochem J. 353(Pt 1):59-67. (PubMedId: 11115399
- Fernandez-Cancio M, Fernandez-Vitos EM, Imperial S, Centelles JJ. Structural requirements of benzodiazepines for the inhibition of pig brain
nitric oxide synthase. (2001) Brain Res Mol Brain Res. 96(1-2):87-93. (PubMedId: 11731013
- Fernandez-Cancio M, Fernandez-Vitos EM, Centelles JJ, Imperial S. Sources of interference in the use of 2,3-diaminonaphthalene for the
fluorimetric determination of nitric oxide synthase activity in biologicalsamples. (2001) Clin Chim Acta. 312(1-2):205-12. (PubMedId: 11580928
- Campos N, Rodriguez-Concepcion M, Seemann M, Rohmer M, Boronat A. Identification of gcpE as a novel gene of the 2-C-methyl-D-erythritol
4-phosphate pathway for isoprenoid biosynthesis in Escherichia coli. (2001) FEBS Lett. 488(3):170-3. (PubMedId: 11163766
- Altincicek B, Moll J, Campos N, Foerster G, Beck E, Hoeffler JF, Grosdemange-Billiard C, Rodriguez-Concepcion M, Rohmer M, Boronat A, Eberl M, Jomaa H. Cutting edge: human gamma delta T cells are activated by intermediates of
the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoidbiosynthesis. (2001) J Immunol. 166(6):3655-8. (PubMedId: 11238603
- Rodriguez-Concepcion M, Ahumada I, Diez-Juez E, Sauret-Gueto S, Lois LM, Gallego F, Carretero-Paulet L, Campos N, Boronat A. 1-Deoxy-D-xylulose 5-phosphate reductoisomerase and plastid isoprenoid
biosynthesis during tomato fruit ripening. (2001) Plant J. 27(3):213-22. (PubMedId: 11532167
- Charon L, Hoeffler JF, Pale-Grosdemange C, Lois LM, Campos N, Boronat A, Rohmer M. Deuterium-labelled isotopomers of 2-C-methyl-D-erythritol as tools for the
elucidation of the 2-C-methyl-D-erythritol 4-phosphate pathway forisoprenoid biosynthesis. (2000) Biochem J. 346 Pt 3:737-42. (PubMedId: 10698701
- Rodriguez-Concepcion M, Campos N, Maria Lois L, Maldonado C, Hoeffler JF, Grosdemange-Billiard C, Rohmer M, Boronat A. Genetic evidence of branching in the isoprenoid pathway for the production
of isopentenyl diphosphate and dimethylallyl diphosphate in Escherichiacoli. (2000) FEBS Lett. 473(3):328-32. (PubMedId: 10818234
- Haba E, Espuny MJ, Busquets M, Manresa A. Screening and production of rhamnolipids by Pseudomonas aeruginosa 47T2
NCIB 40044 from waste frying oils. (2000) J Appl Microbiol. 88(3):379-87. (PubMedId: 10747218
- Lois LM, Rodriguez-Concepcion M, Gallego F, Campos N, Boronat A. Carotenoid biosynthesis during tomato fruit development: regulatory role
of 1-deoxy-D-xylulose 5-phosphate synthase. (2000) Plant J. 22(6):503-13. (PubMedId: 10886770
- Lluch MA, Masferrer A, Arro M, Boronat A, Ferrer A. Molecular cloning and expression analysis of the mevalonate kinase gene
from Arabidopsis thaliana. (2000) Plant Mol Biol. 42(2):365-76. (PubMedId: 10794536
- Cunillera N, Boronat A, Ferrer A. Spatial and temporal patterns of GUS expression directed by 5’ regions of
the Arabidopsis thaliana farnesyl diphosphate synthase genes FPS1 andFPS2. (2000) Plant Mol Biol. 44(6):747-58. (PubMedId: 11202437
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